Pef6/v5-his b

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August undefined, 2024

WebThermo Fisher mammalian expression pef6 v5 his topo plasmid vector Mammalian Expression Pef6 V5 His Topo Plasmid Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. WebpEF6/V5 His TOPO Vector Database Welcome to Vector Database! Vector database is a digital collection of vector backbones assembled from publications and commercially …

pEF6/Myc-His A, B, and C - Thermo Fisher Scientific

WebAll pEF or pUB vectors contain a strong promoter for high-level expression in mammalian cells, a choice of selection marker for generating stable cell lines, and an epitope tag for easy detection with a monoclonal antibody and rapid purification on nickel-chelating resin. Each vector is available in three reading frames to simplify cloning in-frame with the … WebpEF6-V5-His (Search Vector Database) Backbone size w/o insert (bp) 5840 Total vector size (bp) 8009 Vector type Mammalian Expression Growth in Bacteria Bacterial Resistance (s) … own monitor lizard

MembranePro Functional Protein Expression System - Thermo …

Webγ-Aminobutyric acid type A receptors (GABAARs) mediate primarily inhibitory synaptic transmission in the central nervous system. Following fast-paced activation, which provides the selective flow of mainly chloride (Cl−) and less bicarbonate (HCO3−) ions via the pore, these receptors undergo desensitization that is … WebpEF6/V5-His (Search Vector Database) Backbone manufacturer Invitrogen Backbone size w/o insert (bp) 5840 Total vector size (bp) 6531 Vector type Mammalian Expression Selectable markers Blasticidin Growth in Bacteria Bacterial Resistance (s) Ampicillin, 100 μg/mL Growth Temperature 37°C Growth Strain (s) DH5alpha Copy number Unknown … WebpEF6/Myc-His To propagate and maintain the pEF6/Myc-His vectors, we recommend resuspending each vector in 20 μl sterile water to prepare a 1 μg/μl stock solution. Store the stock solution at -20°C. Use the stock solution to transform a recA, endA E. coli strain like TOP10F´, DH5αF´, or equivalent. jedi high council planet

Pef6 Expression Vector Thermo Fisher Bioz

Pef6/v5-his b

WebMoss (National Institutes of Health, Bethesda, MD). The pEF6/V5-His-TOPO® expression vector and the monoclonal mouse anti-V5 antibody were purchased from Invitrogen. Genomic DNA isolated from periph-eral blood lymphocytes of healthy European-, African-, Chinese-, and Hispanic-American volunteers was purchased from Coriell Cell Repos- WebDec 8, 2006 · HEK-293T cells were transfected with pEF6/V5-His recombinant vector containing Lrmp (A), Casc1 (B), Ghiso (C), or Lmna-rs1 (D) full-length cDNAs, grown for 48 h, fixed with paraformaldehyde, and treated with anti-V5 monoclonal antibody followed by secondary antibody-Alexa Fluor 488 conjugate.

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http://www.labbase.net/Supply/SupplyItems-4229347.html WebpEF6/V5-His A, B, and C - Gene Synthesis EN English Deutsch Français Español Português Italiano Român Nederlands Latina Dansk Svenska Norsk Magyar Bahasa Indonesia …

WebJan 15, 2013 · Full-length ADAMTS4 has a protumorigenic effect, whereas full-length E362A inhibits B16 melanoma tumor growth. (a) Expression of ADAMTS4 full-length proteins—WT and E362A mutant in conditioned medium of stably transfected B16 melanoma cell lines using pEF6/V5-His TOPO vector analyzed by immunoblotting with anti-V5 antibody. Blot … WebDensitometric analysis of (A) LRP6 phosphorylation and (B) total LRP6 content in cells treated with the indicated concentrations of Rspo2-2F, Rspo2-2F/Q70R and/or Wnt-3a for 1, 6 or 20 h prior to processing for immunoblotting. Cell lysates also were immunoblotted for HSP70 as a loading control.

WebpEF6 V5-His B Sequence and Map Mammalian vector for high-level constitutive expression of C-terminally V5- and 6xHis-tagged proteins. For other reading frames, use pEF6/V5-His … WebpEF6/V5-His A, B, and C - Gene Synthesis EN English Deutsch Français Español Português Italiano Român Nederlands Latina Dansk Svenska Norsk Magyar Bahasa Indonesia …

WebB I I Pme Comments for pEF6/V5-His A 5818 base pairs EF-1α promoter: bases 474-1651 T7 promoter/priming site: bases 1668-1687 Multiple cloning site: bases 1713-1804 V5 epitope: bases 1805-1846 6xHis tag: bases 1856-1873 BGH reverse priming site: bases 1896-1913

WebThe pEF6/V5-His TOPO™ TA Expression Kit is designed for high-level mammalian expression from the powerful human EF-1 α promoter with the added convenience of one-step TOPO™ Cloning. The pEF6/V5-His … jedi in occupational therapyWebContents 20 μg each pEF6/Myc-His A, B, and C, lyophilized 20 μg pEF6/Myc-His/lacZ, lyophilized Shipping/Storage Lyophilized plasmids are shipped at room temperature and … jedi holding corporationWebThe pEF6/V5-His TOPO™ TA Expression Kit is designed for high-level mammalian expression from the powerful human EF-1 α promoter with the added convenience of one … own motorcycle you have extraWebs871 streptavidin, rhodamine b 1 mg 1627.5. s872 streptavidin, texas red（r 1 mg 1627.5. s888 streptavidin 5 mg 2096.5. s911 streptavidin, horseradish 1 mg 1627.5. s921 streptavidin, alkaline ph 0.5 ml 2612.4. s931 streptavidin, beta-galact 1 mg 2144.8. s951 streptavidin agarose sed 5 ml 2128. sa10001 streptavidin - hrp 50ul 1449. sa10002 ... own my home out right but have bad creditWebpEF6/V5-His A, B, and C - Gene Synthesis EN English Deutsch Français Español Português Italiano Român Nederlands Latina Dansk Svenska Norsk Magyar Bahasa Indonesia Türkçe Suomi Latvian Lithuanian český русский български العربية Unknown own my domainWebDescription pEF-DEST51 is a 7.5 kb vector derived from pEF6/V5-His and adapted for use with the Gateway ™ Technology. It is designed to allow high-level, constitutive expression in a variety of mammalian hosts. Features pEF-DEST51 contains the following elements: • Human elongation factor 1α own my home outright need a loan bad creditWebYÛÈw µˆ Gñ{fµkCÑÅÂ ø¥ m B"ªŒ( MAµÉ8 ÉëN¯ø ª:Æÿì«¿(í“Ê}¤v88«µ ÛùVÒÈFB)l} 5¸ã¤‘â m7ÚM½½ì 5Ä_*Kœ`ô ½ mªÛ dC¥[Þ(¾¶Üí0L ÇÝçëŠÆ R°Ôï Þ Dµ‰eó¥“ Þ{ Î{Ôv¶¢ûW¸ƒ#{ o9r W±þ•Þ ¯PRIü6Ôè~ ÛÍ »4× Ìd„•Éê7v¯W¯4ø q ëÒ Y1 „[ìaƒ¼ ñíŽkÒë’·Äx9 ... jedi journey fimfiction